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Jiraphat Jantharo of the Phi Phi Hotel Group in Krabi, southern Thailand said that most foreigners visiting the holiday island were not wearing masks.
He feared a spike in Covid cases - especially the Omicron variant - and the possibly closure of the island if something is not done fast.
He told the Thai media that foreign tourists were not wearing masks in public, at tourist sites and in convenience stores.
With many places being narrow and congested on the island he feared that spread of the virus would be inevitable.
He called on the Krabi health authorities to take immediate action and fine tourists if necessary.
The comments came after 63 visitors to Krabi tested positive from December 1st to January 1st.
Ten of those had Omicron with a further 27 awaiting confirmation after tests.
The Thai media were at the port in Phi Phi where the main concern was making sure that people were wearing life jackets and boats were not overloaded.
Some 1,300 visitors were there daily with many going to Maya Bay that has recently reopened to tourism.